The effect on thermotolerance of the incompletely dominant frizzle (F) gene, which causes feather curling and feather mass reduction, was investigated in 281 laying hens that were homozygous for the frizzle mutation (FF), heterozygous (FN), or normally feathered (NN). One-half of the birds were kept under standard conditions (22°C) and half were exposed to high ambient temperatures (32°C) between 24 and 46 wk of age. Egg production, egg quality, feed efficiency, and dissection traits were recorded and compared. At standard conditions, egg production and quality traits did not differ among the 3 genotypes, whereas feed efficiency was lower for the homozygous birds. Under heat stress conditions, the superiority of the FF hens was evident for all egg quantity and quality traits. No significant difference was measured between heterozygous carriers and normally feathered hens, indicating that the incomplete dominant frizzle mutation behaved as a recessive mutation regarding heat tolerance. From this study, we deduced that the F mutation in its homozygous state has a beneficial effect in decreasing heat stress in poultry production, and it could be particularly advantageous in tropical countries where average temperatures are never too low to negatively affect feed efficiency.