Abstract
Patchouli alcohol (PA) is a kind of methanol extracted from traditional Chinese medicine Pogostemonis Herba. Our research aimed to observe the anti-influenza virus role of PA in vitro. 16HBE (human respiratory epithelial cell) was infected by H1N1 (A/FM1/1/47) to set the cell model. Then the 16HBE was co-cultivated with three kinds of immune cells: dendritic cells, macrophages, and monocytes, PA (the concentration is 10 μg/mL) was added as a treatment intervention for 24 h. The immune cells and the supernate were collected for RT-PCR and ELISA detection related to RLH (RIG-1-like helicases) pathway. Results showed that the IL-4 and IFN-γ in supernate were increased after H1N1 infection, and the PA treatment suppressed the expression of cytokines and the mRNA of RLH pathway. PA anti-influenza virus may through regulate the RLH singal pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antiviral Agents / pharmacology*
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Dendritic Cells / drug effects
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Dendritic Cells / immunology
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Drugs, Chinese Herbal / pharmacology*
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Humans
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Immunologic Factors / pharmacology*
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Influenza A Virus, H1N1 Subtype / drug effects*
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Influenza A Virus, H1N1 Subtype / physiology
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Influenza, Human / drug therapy
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Influenza, Human / enzymology
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Influenza, Human / immunology*
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Influenza, Human / virology
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Interferon-gamma / genetics
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Interferon-gamma / immunology
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Interleukin-4 / genetics
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Interleukin-4 / immunology
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Macrophages / drug effects
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Macrophages / immunology
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RNA Helicases / genetics
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RNA Helicases / immunology*
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Sesquiterpenes / pharmacology*
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Signal Transduction / drug effects
Substances
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Antiviral Agents
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Drugs, Chinese Herbal
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Immunologic Factors
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Sesquiterpenes
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Interleukin-4
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Interferon-gamma
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RNA Helicases
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patchouli alcohol