Abstract
Five new flavonoid glycosides, namely nervilifordins F-J (1-5), were isolated from the 60% EtOH extract of the aerial parts of Nervilia fordii, along with three first isolated flavonoids (7, 8, and 13) and five known flavonoids (6, 9-12). The structures of new compounds were elucidated on the basis of 1D and 2D NMR and MS studies. Their anti-inflammatory activities were tested by measuring their inhibitory effects on nitric oxide production in lipopolysaccharide-activated RAW264.7 macrophages. Compounds 2 and 5 showed interesting inhibition effects with their EC50 values of 15.15 μM and 14.80 μM, respectively.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Anti-Inflammatory Agents / chemistry
-
Anti-Inflammatory Agents / isolation & purification*
-
Anti-Inflammatory Agents / pharmacology
-
Drugs, Chinese Herbal / chemistry
-
Drugs, Chinese Herbal / isolation & purification*
-
Drugs, Chinese Herbal / pharmacology
-
Flavonoids / chemistry
-
Flavonoids / isolation & purification*
-
Flavonoids / pharmacology
-
Glycosides / chemistry
-
Glycosides / isolation & purification*
-
Glycosides / pharmacology
-
Lipopolysaccharides / pharmacology
-
Macrophages / drug effects
-
Mice
-
Molecular Structure
-
Nitric Oxide / biosynthesis
-
Nuclear Magnetic Resonance, Biomolecular
-
Orchidaceae / chemistry
Substances
-
Anti-Inflammatory Agents
-
Drugs, Chinese Herbal
-
Flavonoids
-
Glycosides
-
Lipopolysaccharides
-
Nitric Oxide
-
3-hydroxyflavone