Abstract
Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans , a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with α- or β-glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (∼58 mol %), mannose (∼22 mol %), and galactose (∼18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue α-D-Glcp-(1→2)-β-D-Galf-(1→6)-α-D-Manp-(1→3)-L-Ser. Terminal α-D-Glcp and (1→6)-β-D-glucan were also detected. The obtained results strongly support the conclusion that these carbohydrates induce defense response in B. napus plants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Ascomycota / chemistry*
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Ascomycota / growth & development
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Ascomycota / immunology
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Aspergillus niger / enzymology
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Brassica napus / drug effects*
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Brassica napus / immunology
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Brassica napus / metabolism
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Brassica napus / microbiology
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Cell Extracts / chemistry
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Cell Extracts / isolation & purification
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Cell Extracts / pharmacology*
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Cell Wall / chemistry*
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Chemistry, Agricultural / methods
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Disease Resistance / drug effects*
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Down-Regulation
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Fungal Proteins / analysis
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Fungal Proteins / chemistry
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Fungal Proteins / metabolism
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Fungicides, Industrial / chemistry
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Fungicides, Industrial / isolation & purification
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Fungicides, Industrial / pharmacology*
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Glycoside Hydrolases / biosynthesis
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Glycoside Hydrolases / genetics
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Glycoside Hydrolases / metabolism
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Glycosides / analysis
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Glycosides / chemistry
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Glycosides / metabolism
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Hydrogen Peroxide / metabolism
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Hydrolysis
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Mycelium / chemistry
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Mycelium / growth & development
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Mycelium / immunology
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Oligosaccharides / analysis
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Oligosaccharides / chemistry
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Oligosaccharides / metabolism
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Peptide Fragments / analysis
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Peptide Fragments / chemistry
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Peptide Fragments / metabolism
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Plant Proteins / biosynthesis
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Seedlings / drug effects
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Seedlings / immunology
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Seedlings / metabolism
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Seedlings / microbiology
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Up-Regulation / drug effects*
Substances
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Cell Extracts
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Fungal Proteins
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Fungicides, Industrial
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Glycosides
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Oligosaccharides
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Peptide Fragments
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Plant Proteins
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pathogenesis-related proteins, plant
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Hydrogen Peroxide
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Glycoside Hydrolases