Abstract
The number of nuclear divisions in meiosis is strictly limited to two. Although the precise mechanism remains unknown, this seems to be achieved by adjusting the anaphase-promoting complex/cyclosome (APC/C) activity to degrade cyclin. Here, we describe a fission yeast cuf2 mutant that enters into a third nuclear division cycle, represented by ectopic spindle assembly and abnormal chromosome segregation. Cuf2 is a meiotic transcription factor, and its critical target is fzr1(+)/mfr1(+), which encodes a meiotic APC/C activator. fzr1Δ also enters a third nuclear division. Thus, Cuf2 ensures termination of the M-phase cycle by boosting Fzr1 expression to generate functional gametes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cdh1 Proteins
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Cell Cycle Proteins / genetics*
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Cell Cycle Proteins / metabolism
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Cell Cycle Proteins / physiology*
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Cyclin B / metabolism
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Gene Expression Regulation, Fungal*
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Gene Knockout Techniques
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Meiosis*
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Schizosaccharomyces / cytology
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Schizosaccharomyces / physiology*
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Schizosaccharomyces pombe Proteins / genetics*
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Schizosaccharomyces pombe Proteins / metabolism
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Schizosaccharomyces pombe Proteins / physiology*
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Single-Cell Analysis
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Spores, Fungal / cytology
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Spores, Fungal / physiology
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Transcription, Genetic
Substances
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Cdc13 protein, S pombe
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Cdh1 Proteins
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Cell Cycle Proteins
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Cuf2 protein, S pombe
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Cyclin B
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Mes1 protein, S pombe
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Schizosaccharomyces pombe Proteins
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mfr1 protein, S pombe