Preparation and electrochemical behavior of L-glutamate electrochemical biosensor

J Biomed Nanotechnol. 2013 Feb;9(2):318-21. doi: 10.1166/jbn.2013.1487.

Abstract

Using the nano-porous pseudo carbon paste electrode (Nano-PPCPE) as the working electrode, mixing L-glutamate oxidase (L-GLOD), catalase (Cat) and bovine serum albumin (BSA) with phosphate buffer (PB, pH = 7.4), followed by cross-linking with glutaraldehyde, a novel L-glutamate electrochemical biosensor was successfully formed. It was demonstrated that the modified nano-PPCPE exhibits a high selectivity and sensitivity in comparison with the modified CPE. The L-glutamate biosensor showed a linear range from 5 x 10(-7) M to 1 x 10(-5) M with the detection limit of 2.5 x 10(-7) M.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • Electrochemical Techniques / methods*
  • Electrodes
  • Glutamic Acid / analysis*
  • Nanoparticles / chemistry
  • Porosity

Substances

  • Glutamic Acid