Phenotyping jasmonate regulation of root growth

Methods Mol Biol. 2013:1011:25-32. doi: 10.1007/978-1-62703-414-2_3.

Abstract

Root architecture is a complex and highly plastic feature of higher plants. Direct treatments with jasmonates and alterations in jasmonate signaling have been shown to elicit a range of root phenotypes. Here, we describe a fast, noninvasive, and semiautomatic method to monitor root architectural responses to environmental stimuli using plant tissue culture and the software tool EZ-RHIZO.

MeSH terms

  • Arabidopsis / anatomy & histology
  • Arabidopsis / growth & development*
  • Culture Media
  • Culture Techniques
  • Cyclopentanes / metabolism*
  • Optical Imaging
  • Oxylipins / metabolism*
  • Phenotype
  • Plant Growth Regulators / physiology*
  • Plant Roots / anatomy & histology
  • Plant Roots / growth & development*
  • Seeds / growth & development
  • Sterilization / methods

Substances

  • Culture Media
  • Cyclopentanes
  • Oxylipins
  • Plant Growth Regulators
  • jasmonic acid