Genotyping of Toxoplasma gondii isolates from wild boars in Peninsular Malaysia

PLoS One. 2013 Apr 17;8(4):e61730. doi: 10.1371/journal.pone.0061730. Print 2013.

Abstract

Toxoplasma gondii is a parasitic protozoan that infects nearly one-third of the world population. The present study was done to isolate and genotype T. gondii from wild boar from forests of Pahang, Malaysia. A total of 30 wild boars' blood, heads and hearts were obtained for this study and 30 (100.0%) were found to be seropositive when assayed with modified agglutination test (MAT ≥ 6). The positive samples were inoculated into mice and T. gondii was only isolated from samples that had strong seropositivity (MAT ≥ 1:24).The isolates were subjected to PCR-RFLP analysis and all the Peninsular Malaysia isolates of T. gondii are of clonal type I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agglutination Tests
  • Animals
  • Genetic Markers
  • Genotyping Techniques*
  • Malaysia
  • Mice
  • Polymorphism, Restriction Fragment Length
  • Sus scrofa / parasitology*
  • Toxoplasma / genetics*
  • Toxoplasma / isolation & purification*
  • Toxoplasmosis, Animal / parasitology*

Substances

  • Genetic Markers

Grants and funding

The study was funded Universiti Sains Malaysia Research University Grant Scheme (1001/CIPPM/813027) and the first author received the National Science Fellowship (MOSTI/BMI/TAJ/1-1/2 Jld. 19 (121)). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.