Abstract
Toxoplasma gondii is a parasitic protozoan that infects nearly one-third of the world population. The present study was done to isolate and genotype T. gondii from wild boar from forests of Pahang, Malaysia. A total of 30 wild boars' blood, heads and hearts were obtained for this study and 30 (100.0%) were found to be seropositive when assayed with modified agglutination test (MAT ≥ 6). The positive samples were inoculated into mice and T. gondii was only isolated from samples that had strong seropositivity (MAT ≥ 1:24).The isolates were subjected to PCR-RFLP analysis and all the Peninsular Malaysia isolates of T. gondii are of clonal type I.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agglutination Tests
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Animals
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Genetic Markers
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Genotyping Techniques*
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Malaysia
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Mice
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Polymorphism, Restriction Fragment Length
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Sus scrofa / parasitology*
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Toxoplasma / genetics*
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Toxoplasma / isolation & purification*
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Toxoplasmosis, Animal / parasitology*
Grants and funding
The study was funded Universiti Sains Malaysia Research University Grant Scheme (1001/CIPPM/813027) and the first author received the National Science Fellowship (MOSTI/BMI/TAJ/1-1/2 Jld. 19 (121)). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.