Objective: To study the chemical constituents from Daphne acutiloba.
Method: The constituents were separated by column chromatography, and their structures were elucidated by spectroscopic data analyses.
Result: Fifteen compounds were isolated from the EtOAc extract and identified as wikstroelide M (1), vesiculosin (2), prostratin (3), 7-hydroxy-coumarin (4), 7,8-di-hydroxy-coumarin (5), isodaphnoside (6), daphnorine (7), rutamontine (8), daphnolin (9), daphneticin (10), (+)-pinoresinol-beta-D-glucoside (11), oleodapnone (12), oleodaphnal (13), ergosterol peroxide (14) and cholest-5-en-3beta-ol (15).
Conclusion: All the compounds except for 4, 5 and 14 were obtained from the stems of this plant for the first time.