Abstract
A single-step concentration and elution method is developed for detection of DNA in buffer, saliva, and blood. A nanotip capturing DNA using an electric field and capillary action is directly dissolved in buffer for qPCR analysis. The concentration yield and the relative parameters are compared with those of a commercial kit.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Analytic Sample Preparation Methods / methods*
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Bacteriophage lambda / genetics
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Carbon Compounds, Inorganic / chemistry
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DNA / analysis
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DNA / blood
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DNA / genetics*
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DNA / isolation & purification*
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DNA, Viral / analysis
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DNA, Viral / genetics
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Genome, Human / genetics
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Humans
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Nanotechnology / methods*
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Nanowires / chemistry
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Polymerase Chain Reaction / methods*
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Saliva / chemistry
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Silicon Compounds / chemistry
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Time Factors
Substances
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Carbon Compounds, Inorganic
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DNA, Viral
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Silicon Compounds
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DNA
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silicon carbide