Objective: To predict and identify Taenia solium oncosphere TSO45-4B antigen Fn III structure domain linear B cell epitopes.
Methods: The B cell epitopes of TSO45-4B Fn III structure domain were predicted through the sequence analysis by using bioinformatics online tools and the protein space conformation was predicted by SWISS-MODEL. The peptides were synthesized according to the predicted linear epitopes. The immunoreactivity of sera of cysticercosis patients to the peptides synthesized was tested by using ELISA.
Results: Two linear B cell epitopes of TSO45-4B Fn III structure domain were predicted, and one of the predicted epitope peptides synthesized could be recognized by the sera of cysticercosis patients.
Conclusion: Two linear B cell epitopes of TSO45-4B Fn III structure domain are predicted and one of them has been confirmed successfully.