A new multichannel spectral imaging laser scanning confocal microscope

Comput Math Methods Med. 2013:2013:890203. doi: 10.1155/2013/890203. Epub 2013 Mar 26.

Abstract

We have developed a new multichannel spectral imaging laser scanning confocal microscope for effective detection of multiple fluorescent labeling in the research of biological tissues. In this paper, the design and key technologies of the system are introduced. Representative results on confocal imaging, 3-dimensional sectioning imaging, and spectral imaging are demonstrated. The results indicated that the system is applicable to multiple fluorescent labeling in biological experiments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA / metabolism
  • Diagnostic Imaging / methods
  • Equipment Design
  • Fluorescent Dyes / pharmacology
  • Image Processing, Computer-Assisted / methods
  • Imaging, Three-Dimensional / methods
  • Indoles / pharmacology
  • Kidney / pathology
  • Lasers*
  • Mice
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence / methods
  • Optics and Photonics / methods
  • Rats

Substances

  • Fluorescent Dyes
  • Indoles
  • DAPI
  • DNA