Extraction of DNA staining dyes from DNA using hydrophobic ionic liquids

Imran Khimji; Krystina Doan; Kiara Bruggeman; Po-Jung Jimmy Huang; Puja Vajha; Juewen Liu

doi:10.1039/c3cc41364k

Extraction of DNA staining dyes from DNA using hydrophobic ionic liquids

Chem Commun (Camb). 2013 May 18;49(40):4537-9. doi: 10.1039/c3cc41364k. Epub 2013 Apr 11.

Authors

Imran Khimji¹, Krystina Doan, Kiara Bruggeman, Po-Jung Jimmy Huang, Puja Vajha, Juewen Liu

Affiliation

¹ Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

PMID: 23575619
DOI: 10.1039/c3cc41364k

Abstract

The partition of oligonucleotides and DNA staining dyes into a few hydrophobic ionic liquids has been studied, where the oligonucleotides remain in the aqueous phase and all the DNA staining dyes are extracted in the ionic liquid phase, allowing the separation of these two.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / isolation & purification*
Fluorescence
Fluorescent Dyes / isolation & purification*
Hydrophobic and Hydrophilic Interactions
Ionic Liquids / chemistry*

Substances

Fluorescent Dyes
Ionic Liquids
DNA