Profiling glycosyltransferase activities by tritium imaging of glycan microarrays

Chembiochem. 2013 May 10;14(7):862-9. doi: 10.1002/cbic.201300051. Epub 2013 Apr 8.

Abstract

High-throughput microarray technology has been combined with ultrasensitive and high-resolution tritium autoradiography to create a new platform for the quantitative detection of glycosyltransferase activity on glycan arrays. In addition, we show full compatibility with the use of fluorescently labeled lectins to help with the stereochemical assignment of newly formed glycoside linkages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Enzyme Activation
  • Glycosyltransferases / analysis
  • Glycosyltransferases / metabolism*
  • Microarray Analysis*
  • Molecular Sequence Data
  • Polysaccharides / metabolism*
  • Schistosomiasis mansoni / enzymology
  • Tritium / analysis*
  • Tritium / metabolism

Substances

  • Polysaccharides
  • Tritium
  • Glycosyltransferases