Sonoporation: mechanistic insights and ongoing challenges for gene transfer

Anthony Delalande; Spiros Kotopoulis; Michiel Postema; Patrick Midoux; Chantal Pichon

doi:10.1016/j.gene.2013.03.095

Sonoporation: mechanistic insights and ongoing challenges for gene transfer

Gene. 2013 Aug 10;525(2):191-9. doi: 10.1016/j.gene.2013.03.095. Epub 2013 Apr 6.

Authors

Anthony Delalande¹, Spiros Kotopoulis, Michiel Postema, Patrick Midoux, Chantal Pichon

Affiliation

¹ Centre de Biophysique Moléculaire, CNRS, Orléans, France.

PMID: 23566843
DOI: 10.1016/j.gene.2013.03.095

Abstract

Microbubbles first developed as ultrasound contrast agents have been used to assist ultrasound for cellular drug and gene delivery. Their oscillation behavior during ultrasound exposure leads to transient membrane permeability of surrounding cells, facilitating targeted local delivery. The increased cell uptake of extracellular compounds by ultrasound in the presence of microbubbles is attributed to a phenomenon called sonoporation. In this review, we summarize current state of the art concerning microbubble-cell interactions and cellular effects leading to sonoporation and its application for gene delivery. Optimization of sonoporation protocol and composition of microbubbles for gene delivery are discussed.

Keywords: 1,2-dimyristoyl-sn-glycero-3-phosphocholine; 1,2-distearoyl-sn-glycero-3-phosphocholine; DMPC; DSPC; Gene therapy; HIFU; High Intensity Focused Ultrasound; Microbubbles; Physical gene delivery method; Ultrasound; VEGF; Vascular Endothelium Growth Factor; eGFP; enhanced Green Fluorescent Protein.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Cell Membrane Permeability
Drug Delivery Systems / methods*
Gene Transfer Techniques*
Humans
Microbubbles
Ultrasonics*