A robust and efficient method for the isolation of DNA-free, pure and intact RNA from Mycobacterium tuberculosis

Balaji Venkataraman; Nidhi Gupta; Amita Gupta

doi:10.1016/j.mimet.2013.03.018

A robust and efficient method for the isolation of DNA-free, pure and intact RNA from Mycobacterium tuberculosis

J Microbiol Methods. 2013 Jun;93(3):198-202. doi: 10.1016/j.mimet.2013.03.018. Epub 2013 Apr 6.

Authors

Balaji Venkataraman¹, Nidhi Gupta, Amita Gupta

Affiliation

¹ Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.

PMID: 23566823
DOI: 10.1016/j.mimet.2013.03.018

Abstract

We describe a robust method for the isolation of pure, intact RNA suitable for transcriptome studies from mycobacteria with consistent yields of 1 μg to 3 μg total RNA per 10(7) cells. The method reduces the use of hazardous chemicals and incorporates protocols for efficient removal of gDNA and rRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques / methods*
Gene Expression Profiling / methods
Molecular Biology / methods*
Mycobacterium tuberculosis / genetics*
RNA, Bacterial / isolation & purification*
Specimen Handling / methods*

Substances

RNA, Bacterial