Abstract
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of PKR, substantially increased production of recombinant TPL-2 (tumour progression locus 2)-ABIN-2 [A20-binding inhibitor of NF-κB (nuclear factor κB) 2]-NF-κB1 p105 complex. VP35 also increased expression of other co-transfected proteins, suggesting that VP35 could be employed generally to boost recombinant protein production by HEK-293 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / biosynthesis*
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Adaptor Proteins, Signal Transducing / genetics
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Ebolavirus / genetics
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Ebolavirus / physiology*
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HEK293 Cells
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Humans
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MAP Kinase Kinase Kinases / biosynthesis*
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MAP Kinase Kinase Kinases / genetics
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Multiprotein Complexes / biosynthesis
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Multiprotein Complexes / genetics
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NF-kappa B p50 Subunit / biosynthesis*
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NF-kappa B p50 Subunit / genetics
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Proto-Oncogene Proteins / biosynthesis*
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Proto-Oncogene Proteins / genetics
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Transfection
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Up-Regulation / genetics*
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Viral Regulatory and Accessory Proteins / genetics
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Viral Regulatory and Accessory Proteins / physiology*
Substances
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Adaptor Proteins, Signal Transducing
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Multiprotein Complexes
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NF-kappa B p50 Subunit
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NFKB1 protein, human
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Proto-Oncogene Proteins
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Recombinant Proteins
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TNIP2 protein, human
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VP35 protein, filovirus
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Viral Regulatory and Accessory Proteins
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MAP Kinase Kinase Kinases
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MAP3K8 protein, human