Abstract
A method based on electrogenerated chemiluminescence (ECL) for detection of DNA methylation and assay of the methyltransferase activity is developed, and it is demonstrated that the label-free ECL method is capable of detecting methyltransferase with a detection limit of 3 × 10(-6) U mL(-1), using a supersandwich amplification technique.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biosensing Techniques*
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DNA / chemistry*
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DNA Methylation*
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DNA-Cytosine Methylases / analysis*
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DNA-Cytosine Methylases / chemistry*
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Electrochemical Techniques
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Electrodes
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Limit of Detection
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Luminescent Measurements / methods*
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Organometallic Compounds / chemistry
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Phenanthrolines / chemistry
Substances
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Organometallic Compounds
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Phenanthrolines
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tris-(1,10-phenanthroline)ruthenium
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DNA
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DNA modification methylase HpaII
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DNA modification methylase SssI
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DNA-Cytosine Methylases