A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity

Chem Commun (Camb). 2013 May 10;49(37):3869-71. doi: 10.1039/c3cc00203a.

Abstract

A method based on electrogenerated chemiluminescence (ECL) for detection of DNA methylation and assay of the methyltransferase activity is developed, and it is demonstrated that the label-free ECL method is capable of detecting methyltransferase with a detection limit of 3 × 10(-6) U mL(-1), using a supersandwich amplification technique.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques*
  • DNA / chemistry*
  • DNA Methylation*
  • DNA-Cytosine Methylases / analysis*
  • DNA-Cytosine Methylases / chemistry*
  • Electrochemical Techniques
  • Electrodes
  • Limit of Detection
  • Luminescent Measurements / methods*
  • Organometallic Compounds / chemistry
  • Phenanthrolines / chemistry

Substances

  • Organometallic Compounds
  • Phenanthrolines
  • tris-(1,10-phenanthroline)ruthenium
  • DNA
  • DNA modification methylase HpaII
  • DNA modification methylase SssI
  • DNA-Cytosine Methylases