Substitutions T200A and E227A in the hemagglutinin of pandemic 2009 influenza A virus increase lethality but decrease transmission

J Virol. 2013 Jun;87(11):6507-11. doi: 10.1128/JVI.00262-13. Epub 2013 Mar 27.

Abstract

We report that swine influenza virus-like substitutions T200A and E227A in the hemagglutinin (HA) of the 2009 pandemic influenza virus alter its pathogenesis and transmission. Viral replication is increased in mammalian cells. Infected mice show increased disease as measured by weight loss and lethality. Transmission in ferrets is decreased in the presence of both substitutions, suggesting that amino acids 200T and 227E are adaptive changes in the HA of swine origin influenza viruses associated with increased transmission and decreased pathogenesis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Substitution*
  • Animals
  • Down-Regulation
  • Female
  • Ferrets
  • Hemagglutinin Glycoproteins, Influenza Virus / chemistry
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics*
  • Hemagglutinin Glycoproteins, Influenza Virus / metabolism
  • Humans
  • Influenza A Virus, H1N1 Subtype / chemistry
  • Influenza A Virus, H1N1 Subtype / genetics*
  • Influenza A Virus, H1N1 Subtype / metabolism
  • Influenza A Virus, H1N1 Subtype / pathogenicity*
  • Influenza, Human / epidemiology
  • Influenza, Human / mortality*
  • Influenza, Human / transmission*
  • Influenza, Human / virology
  • Mice
  • Mice, Inbred DBA
  • Pandemics
  • Up-Regulation
  • Virulence

Substances

  • Hemagglutinin Glycoproteins, Influenza Virus