Abstract
Here we report the design and evaluation of a loop-mediated isothermal amplification (LAMP) assay for detecting Trueperella pyogenes DNA based on the plo gene sequence that encodes pyolysin. The results showed that target DNA was amplified specifically and with a detection limit 100-fold greater than PCR.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinomycetaceae / genetics
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Actinomycetaceae / isolation & purification*
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Bacterial Proteins / genetics
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Bacterial Toxins / genetics
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Bacteriological Techniques / methods*
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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Hemolysin Proteins / genetics
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Nucleic Acid Amplification Techniques / methods*
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Sensitivity and Specificity
Substances
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Bacterial Proteins
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Bacterial Toxins
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DNA, Bacterial
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Hemolysin Proteins
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Pyolysin, Arcanobacterium pyogenes