Development of a "signal-on" electrochemical DNA sensor with an oligo-thymine spacer for point mutation detection

Chem Commun (Camb). 2013 Apr 28;49(33):3422-4. doi: 10.1039/c3cc41281d.

Abstract

We report a reagentless and reusable "signal-on" electrochemical DNA sensor that utilizes oligo-thymine as a flexible spacer. This sensor design is sensitive, specific, and selective; it is potentially generalizable for detection of a wide range of biomedical-relevant nucleic acid sequences.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biosensing Techniques
  • DNA / analysis*
  • DNA / genetics
  • DNA / metabolism
  • DNA Probes / chemistry
  • DNA Probes / metabolism
  • Electrochemical Techniques*
  • Electrodes
  • Gold / chemistry
  • Methylene Blue / metabolism
  • Nucleic Acid Hybridization
  • Oligonucleotides / chemistry*
  • Point Mutation
  • Thymine / chemistry*

Substances

  • DNA Probes
  • Oligonucleotides
  • Gold
  • DNA
  • Thymine
  • Methylene Blue