Determination of low urinary 8-hydroxy-2`-deoxyguanosine excretion with capillary electrophoresis and molecularly imprinted monolith solid phase microextraction

Abstract

As a sensitive biomarker, the level of 8-hydroxy-2`-deoxyguanosine (8-OHdG) indicates the extent of DNA oxidative damage of subject. However its determination in human urine is confounded by trace content and a complex matrix. In this study, a new approach of solid phase microextraction (SPME) coupled to capillary electrophoresis and electrochemical detection was developed with molecularly imprinted monolithic column as extraction sorbent. The tailor made monolith exhibited high extraction efficiency with the enrichment factor 73 for 8-OHdG owing to its special porous structure and inherent selectivity. Under optimal conditions, appreciable sensitivity had been achieved with a limit of detection of 2.61 nmol/L (signal/noise=3) and a limit of quantification of 8.63 nmol/L (signal/noise=10), respectively. As an alternative of precise 8-OHdG determination, wide range linearity had been achieved with 0.01-1.50 μmol/L and RSDs with 3.70% for migration time and 4.80% for peak current (10-1000 nmol/L). 106 samples with relative low extents of DNA oxidative damage were collected and analyzed which including coke plant workers, traffic policemen, taxi drivers and healthy students. Elevated urinary 8-OHdG excretions of subjects may correlate to working condition, occupational exposure, or lifestyle.