Monitoring fast reactions by spatially-selective and frequency-shifted continuous NMR spectroscopy: application to rapid-injection protein unfolding

Chem Commun (Camb). 2013 Apr 18;49(30):3155-7. doi: 10.1039/c3cc39107h. Epub 2013 Mar 12.

Abstract

The repetition rate of an NMR experiment is usually limited by the longitudinal relaxation times of the investigated molecule. Here we show that continuous excitation and data acquisition, without any interscan delay, is possible by a spatially resolved experiment where different nuclei are excited in consecutive scans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Nuclear Magnetic Resonance, Biomolecular*
  • Protein Unfolding*
  • Proteins / chemistry*
  • Proteins / metabolism

Substances

  • Proteins