Absorption measurements in liquid core waveguides using cavity ring-down spectroscopy

Anal Chem. 2013 May 7;85(9):4328-34. doi: 10.1021/ac4007073. Epub 2013 Apr 8.

Abstract

Short liquid core waveguides (LCWs) were included into a fiber-loop cavity ring-down absorption spectrometer to reduce the detection limit over, both, single pass absorption in a LCW and cavity-enhanced absorption using a conventional fiber-loop cavity. LCWs of 5 and 10 cm length were interfaced with a pressure-flow system and a multimode fiber-loop cavity using concave fiber lenses with matching numerical apertures and diameters. Two red dyes, Allura Red AC and Congo Red, were detected with a 532 nm pulsed laser at a 5 nM limit of detection in a detection volume of less than 1 μL, corresponding to a minimal detectable absorbance of less than 4 × 10(-4) cm(-1) and a minimal detectable change in absorption cross section, σ(min) = V(det) × ε × C(LOD), of about 14 μm(2) (Allura Red AC) and 37 μm(2) (Congo Red).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cobalt / analysis*
  • DNA / analysis*
  • Ferrous Compounds / chemistry*
  • Lasers
  • Organic Chemicals / analysis*
  • Potassium Permanganate / analysis*
  • Silicon Dioxide / chemistry
  • Spectrometry, Fluorescence

Substances

  • Ferrous Compounds
  • Organic Chemicals
  • Potassium Permanganate
  • Cobalt
  • Silicon Dioxide
  • DNA
  • cobaltous chloride