Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches

Qian Cheng; F Peter Guengerich

doi:10.1007/978-1-62703-321-3_6

Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches

Methods Mol Biol. 2013:987:71-7. doi: 10.1007/978-1-62703-321-3_6.

Authors

Qian Cheng¹, F Peter Guengerich

Affiliation

¹ Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN, USA.

Abstract

Metabolomics provides an invaluable means to interrogate the function of "orphan" enzymes, i.e., those whose endogenous substrates are not known. Here we describe a high-performance liquid chromatography-coupled mass spectrometry (HPLC-MS)-based metabolomics approach to identify an endogenous substrate of an orphan cytochrome P450.

MeSH terms

Animals
Chromatography, High Pressure Liquid
Cytochrome P-450 Enzyme System / metabolism*
Mass Spectrometry
Metabolomics / methods*
Protein Binding
Software

Substances

Cytochrome P-450 Enzyme System

Abstract

MeSH terms

Substances

Grants and funding