A single-molecule force spectroscopy study of the interactions between lectins and carbohydrates on cancer and normal cells

Weidong Zhao; Mingjun Cai; Haijiao Xu; Junguang Jiang; Hongda Wang

doi:10.1039/c3nr00553d

A single-molecule force spectroscopy study of the interactions between lectins and carbohydrates on cancer and normal cells

Nanoscale. 2013 Apr 21;5(8):3226-9. doi: 10.1039/c3nr00553d. Epub 2013 Mar 11.

Authors

Weidong Zhao¹, Mingjun Cai, Haijiao Xu, Junguang Jiang, Hongda Wang

Affiliation

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.

PMID: 23475354
DOI: 10.1039/c3nr00553d

Abstract

The interaction forces between carbohydrates and lectins were investigated by single-molecule force spectroscopy on both cancer and normal cells. The binding kinetics was also studied, which shows that the carbohydrate-lectin complex on cancer cells is less stable than that on normal cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Surface / chemistry
Dogs
Glucose / chemistry*
HeLa Cells
Humans
Madin Darby Canine Kidney Cells
Mannose / chemistry*
Microscopy, Atomic Force / methods*
Neoplasms / chemistry*
Protein Binding
Spectrum Analysis / methods*
Wheat Germ Agglutinins / chemistry*

Substances

Antigens, Surface
Wheat Germ Agglutinins
Glucose
Mannose