Chemical ligation of oligodeoxynucleotides by X-irradiation and its application to regulation of G-quadruplex formation

Kazuhito Tanabe; Taiga Mizoe; Masaaki Sugiura; Kana Okada; Sei-ichi Nishimoto

doi:10.1016/j.bmcl.2013.01.123

Chemical ligation of oligodeoxynucleotides by X-irradiation and its application to regulation of G-quadruplex formation

Bioorg Med Chem Lett. 2013 Apr 1;23(7):2098-100. doi: 10.1016/j.bmcl.2013.01.123. Epub 2013 Feb 8.

Authors

Kazuhito Tanabe¹, Taiga Mizoe, Masaaki Sugiura, Kana Okada, Sei-ichi Nishimoto

Affiliation

¹ Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan. tanabeka@scl.kyoto-u.ac.jp

PMID: 23465613
DOI: 10.1016/j.bmcl.2013.01.123

Abstract

We demonstrated radiolytic ligation of oligodeoxynucleotides (ODNs) possessing disulfide bond and its application to regulation of DNA quadruplex formation. G-rich hexamer ODNs had poor ability to form quadruplex, while X-irradiation of the ODNs induced interstrand exchange reaction at disulfide bond to form ligated 12 mer ODNs, leading to the ready formation of quadruplex due to the entropic effect. Since complexation of the ligated ODNs with hemin in the presence of K(+) showed strong soret band absorption and also catalyzed the H(2)O(2)-mediated oxidation of luminol, it appears that the quadruplex formed from ligated ODNs showed a function similar to native DNA quadruplex.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry*
G-Quadruplexes*
Nucleic Acid Conformation
Oligodeoxyribonucleotides / chemistry*
X-Rays

Substances

Oligodeoxyribonucleotides
DNA