Abstract
Using selective receptor's agonist and antagonists we show that mouse white fat cells express alpha1A-, alpha2-adrenergic receptors, which activation with noradrenaline is capable of causing calcium responses different by formation mechanism. Adipocyte's calcium responses to alpha1-adrenoreceptor agonists are caused by alpha1A-type adrenoreceptor and suppressed by inhibitors of PLC-dependent pathway. Calcium responses to alpha2-adrenoreceptors agonists are realized only in the presence of more than 200 microM of L-arginine and suppressed by inhibitors of NOS-PKG-RyR pathway. The incubation of cells with L-arginine creates conditions for switching on the signal pathway with participation of eNOS --> NO --> sGC --> cGMP --> PKG --> CD38 --> RyR --> Ca2+ and for switching of the PLC - IP3R-dependent pathway. Adipocyte's calcium response to L-arginine represents a sharp impulse of the big amplitude and is mediated by alpha2-adrenoreceptors. L-arginine activating alpha2-adrenoreceptors and being the substrate of eNOS, realizes two functions in this pathway.
Publication types
-
English Abstract
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adipocytes / cytology
-
Adipocytes / drug effects*
-
Adipocytes / metabolism
-
Adipose Tissue, White / cytology
-
Adipose Tissue, White / drug effects
-
Adipose Tissue, White / metabolism
-
Adrenergic Agonists / pharmacology*
-
Adrenergic Antagonists / pharmacology*
-
Animals
-
Arginine / metabolism
-
Arginine / pharmacology
-
Calcium / metabolism*
-
Calcium Signaling / drug effects*
-
Calcium Signaling / physiology
-
Cell Differentiation
-
Enzyme Inhibitors / pharmacology
-
Imidazoles / pharmacology
-
Mice
-
Nitric Oxide / metabolism
-
Nitric Oxide Synthase Type III / metabolism
-
Norepinephrine / pharmacology*
-
Phenylephrine / pharmacology
-
Primary Cell Culture
-
Receptors, Adrenergic, alpha-1 / metabolism
-
Receptors, Adrenergic, alpha-2 / metabolism*
-
Tetrahydronaphthalenes / pharmacology
-
Type C Phospholipases / metabolism
Substances
-
A 61603
-
Adrenergic Agonists
-
Adrenergic Antagonists
-
Enzyme Inhibitors
-
Imidazoles
-
Receptors, Adrenergic, alpha-1
-
Receptors, Adrenergic, alpha-2
-
Tetrahydronaphthalenes
-
Phenylephrine
-
Nitric Oxide
-
Arginine
-
Nitric Oxide Synthase Type III
-
Nos3 protein, mouse
-
Type C Phospholipases
-
Calcium
-
Norepinephrine