Abstract
Alternative delivery entities are desirable in immunotherapies in which polyplexes are widely formed by electrostatic interactions to induce cellular uptake processes for bioactive molecules. In our study, biocompatible Ni(II)-nitrilo(triacetic acid)-modified poly(ethylene imine)-maltose (Ni-NTA-DG) is realized and evaluated as complexation agent against His-tagged peptides using fluorescence polarization and dynamic light scattering. The polyplexes are stable until a pH of 6.5-6.0, and also up to 50 mM of imidazole. A first uptake approach shows that polyplexes lead to an increase in peptide uptake in monocyte-derived immature dendritic cells. In summary, Ni-NTA-DG represents a promising (delivery) platform for forthcoming in vitro applications.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cell Death / drug effects
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Cell Differentiation / drug effects
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Chemical Phenomena*
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Dendritic Cells / cytology
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Dendritic Cells / drug effects
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Dendritic Cells / metabolism
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HIV / chemistry*
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Humans
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Imines / chemical synthesis
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Imines / chemistry
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Imines / pharmacology*
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Imines / toxicity
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Light
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Maltose / chemistry
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Maltose / pharmacology*
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Molecular Sequence Data
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Nickel / pharmacology*
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Nitrilotriacetic Acid / chemical synthesis
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Nitrilotriacetic Acid / chemistry
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Nitrilotriacetic Acid / toxicity
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Peptides / chemistry
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Peptides / pharmacology*
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Polyethylenes / chemical synthesis
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Polyethylenes / chemistry
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Polyethylenes / pharmacology*
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Polyethylenes / toxicity
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Scattering, Radiation
Substances
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Imines
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Peptides
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Polyethylenes
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poly(ethylene imine)
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Maltose
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Nickel
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Nitrilotriacetic Acid