Ni(II)-NTA modified poly(ethylene imine) glycopolymers: physicochemical properties and first in vitro study of polyplexes formed with HIV-derived peptides

Macromol Biosci. 2013 May;13(5):531-8. doi: 10.1002/mabi.201200449. Epub 2013 Mar 4.

Abstract

Alternative delivery entities are desirable in immunotherapies in which polyplexes are widely formed by electrostatic interactions to induce cellular uptake processes for bioactive molecules. In our study, biocompatible Ni(II)-nitrilo(triacetic acid)-modified poly(ethylene imine)-maltose (Ni-NTA-DG) is realized and evaluated as complexation agent against His-tagged peptides using fluorescence polarization and dynamic light scattering. The polyplexes are stable until a pH of 6.5-6.0, and also up to 50 mM of imidazole. A first uptake approach shows that polyplexes lead to an increase in peptide uptake in monocyte-derived immature dendritic cells. In summary, Ni-NTA-DG represents a promising (delivery) platform for forthcoming in vitro applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Death / drug effects
  • Cell Differentiation / drug effects
  • Chemical Phenomena*
  • Dendritic Cells / cytology
  • Dendritic Cells / drug effects
  • Dendritic Cells / metabolism
  • HIV / chemistry*
  • Humans
  • Imines / chemical synthesis
  • Imines / chemistry
  • Imines / pharmacology*
  • Imines / toxicity
  • Light
  • Maltose / chemistry
  • Maltose / pharmacology*
  • Molecular Sequence Data
  • Nickel / pharmacology*
  • Nitrilotriacetic Acid / chemical synthesis
  • Nitrilotriacetic Acid / chemistry
  • Nitrilotriacetic Acid / toxicity
  • Peptides / chemistry
  • Peptides / pharmacology*
  • Polyethylenes / chemical synthesis
  • Polyethylenes / chemistry
  • Polyethylenes / pharmacology*
  • Polyethylenes / toxicity
  • Scattering, Radiation

Substances

  • Imines
  • Peptides
  • Polyethylenes
  • poly(ethylene imine)
  • Maltose
  • Nickel
  • Nitrilotriacetic Acid