Objective: To explore the expression of SH2B1 adaptor protein in oesophageal cancer and its clinical significance.
Methods: SH2B1 expression in tissue specimens of 120 primary oesophageal cancers, tissues of 120 paired adjacent non-cancer and another 120 normal tissues was analyzed by immunohistochemical SABC staining and Western blot. SH2B1 expression in the oesophageal cancer tissues was analyzed with clinicopathological parameters. SH2B1 expression of normal human esophageal epithelial cells (HEEC) and 2 oesophageal cancer cell lines, TE-1 and Eca109, were evaluated by RT-PCR and Western blot.
Results: SH2B1 expression in the normal oesophageal tissues, adjacent non-cancer tissues and cancer tissues was gradually increased (P<0.05), and significantly associated with the depth of invasion, clinical TNM stage, lymph node metastasis (P<0.05), but it did not have significant association with gender, age, drinking, type and grade of tumor (P>0.05). SH2B1 expression was detectable in all cell lines by RT-PCR and Western blot, but the expression in the two oesophageal cancer cell lines was significantly higher than that in the normal HEEC.
Conclusions: Over-expression of SH2B1 might play an important role in the occurrence and development of human oesophageal cancer and closely correlate with malignant progression of invasion and metastasis of oesophageal cancer.