Effects of ectopic HER-2/neu gene expression on the COX-2/PGE2/P450arom signaling pathway in endometrial carcinoma cells: HER-2/neu gene expression in endometrial carcinoma cells

J Exp Clin Cancer Res. 2013 Mar 2;32(1):11. doi: 10.1186/1756-9966-32-11.

Abstract

Objectives: To investigate the role of HER-2/neu-mediated COX-2/P450arom signal in estrogen-dependent endometrial carcinoma.

Methods: The recombinant eukaryotic expression vector, pcDNA3.1-HER-2/neu, was constructed and transfect to Ishikawa endometrial carcinoma cells. The expression of COX-2 and P450arom in transfected cells were detected by real-time PCR and western blotting. The levels of estrogen in cell supernatants were detected by ELISA.

Results: Over-expression of HER-2/neu in transfected cells was confirmed by real-time PCR and western blotting. The levels of autocrine estrogen in transfected cells was significantly increased which combination with the enhancement of COX-2 and P450arom expression in transfected cells.

Conclusion: HER-2/neu induced the improvement of autocrine estrogen in endometrial carcinoma cell through triggering the COX-2/P450arom signal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aromatase / genetics
  • Aromatase / metabolism*
  • Cyclooxygenase 2 / genetics
  • Cyclooxygenase 2 / metabolism*
  • Dinoprostone / genetics
  • Dinoprostone / metabolism*
  • Endometrial Neoplasms / enzymology
  • Endometrial Neoplasms / genetics*
  • Endometrial Neoplasms / metabolism
  • Female
  • Gene Expression
  • Genes, erbB-2*
  • Humans
  • Receptor, ErbB-2 / biosynthesis*
  • Receptor, ErbB-2 / genetics
  • Signal Transduction
  • Transfection

Substances

  • Aromatase
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • ERBB2 protein, human
  • Receptor, ErbB-2
  • Dinoprostone