Abstract
The benzoylformate decarboxylase gene (mdlC) from Pseudomonas putida was expressed in Escherichia coli BL21(DE3). The recombinant strain together with E. coli/pET30a-mdlB converted (S)-3-ethoxy-4-hydroxymandelic acid (S-EMA) into ethyl vanillin without ethyl vanillin degradation. 4 g ethyl vanillin/l was obtained from 10 g EMA/l within 12 h at 30 °C. This is the first report on the biotransformation of (S)-EMA to ethyl vanillin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzaldehydes / metabolism*
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Biotransformation
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Carboxy-Lyases / genetics
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Carboxy-Lyases / metabolism*
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Decarboxylation
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Mandelic Acids / metabolism*
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Metabolic Engineering*
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Pseudomonas putida / enzymology*
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Pseudomonas putida / genetics
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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Benzaldehydes
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Mandelic Acids
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Recombinant Proteins
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Carboxy-Lyases
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benzoylformate decarboxylase
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mandelic acid
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ethyl vanillin