A comprehensive ecosystem approach-based surveillance system was designed and implemented for dengue prevention in Cotorro municipality at the City of Havana. Cuba, geographically situated near those countries with high dengue incidence, and having a preventive approach as a premise of its public healthcare system, has to adopt measures to prevent new dengue epidemics. The environmental, entomological and clinical/epidemiological elements of the surveillance system were integrated and combined with a social participation strategy. A number of workshops were held for the people involved in search and analysis of the collected information. An automated database with indicators and thematic map outputs made risk stratification for dengue and its vector possible. Additionally, 17 groups of neighbors were organized. The environmental surveillance was the first element to be taken into account to avoid Aedes aegypti spread. The comprehensive surveillance system for dengue developed in the project was an important tool for the decision-taking process at local level.