Intensity of neoangiogenesis depends on relationship between factors that are responsible for stimulation and inhibition of this process. The intensity of neoangiogenesis could be assessed by the microvasculature density (MVD). Recent reports highlight the role of glycoprotein CD105 (endoglin) in the neoangiogenesis. CD105 is a transmembrane glycoprotein, which is a receptor for transforming growth factor. CD105 is overexpressed in the endothelium of blood vessels of tissues, particularly in de novo formed blood vessels within tumor. The aim of this study was to evaluate the microvesseldensity by changes in CD105 expression in laryngeal squamous cell carcinoma (SCC). Expression of CD105 protein was performed using automated morphometric methods. New blood vessels density was demonstrated by CD105 expression as (MDV/CD105), and performed using the authors' "macro" function based on 'color deconvolation' and 'manual threshold" in the ImageJ program. Analysis of average microvessel density was performed in three hot spots, and includes the area of positively stained endothelial cells (0-100), level of CD105 expression (0-255). We have correlated the staining patterns with clinicopathological data. Analysis of CD105 expression revealed higher values MVD/CD105 in squamous cell carcinoma than in normal mucosa 115.44 vs. 55.83. Moreover, microvessel density was higher in N(+) group MVD/CD105 = 144.72 (SD = 104.15) than in the N(0) group MVD/CD105 80.44 (SD = 68.57). Analysis of correlation of CD105 expression in N(0) vs. N(+) demonstrated a statistically significant difference (p = 0.000007). MVD/CD105 may be important prognostic factor in laryngeal squamous cell carcinoma.