Rapid determination of α-tocopherol in cereal grains using dispersive liquid-liquid microextraction followed by HPLC

Abstract

The traditional for the determination of α-tocopherol in cereal grains includes saponification of a sample followed by liquid-liquid extraction, and it is time- and solvent consuming. In this study, a dispersive liquid-liquid microextraction (DLLME) method was developed to extract α-tocopherol in situ from the saponified grain sample solution. The DLLME experimental parameters including the type and volume of extractants, the volume of dispersers, the addition of salt and the extraction/centrifuging time were examined and optimized. The recommended analytical procedure showed excellent precision (relative SDs of the α-tocopherol amount of 3.1% over intraday and 7.2% over interday), high sensitivity (the detection limit of 1.9 ng/mL), and strong recovery values (88.9-102.5%). In addition, statistical analyses showed no significant difference between the detected amounts of α-tocopherol found by the standardized method and this new procedure. The method was successfully applied to determining the amounts and distribution of α-tocopherol in 14 cereal grain samples.