Abstract
Objective:
To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM).
Materials and methods:
Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specific PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups.
Results:
MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a significant difference in MGMT methylation between the two groups (p < 0.05).
Conclusions:
We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / genetics*
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Adaptor Proteins, Signal Transducing / metabolism
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Adult
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China / epidemiology
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Chromosomes, Human / drug effects*
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Chromosomes, Human / genetics
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DNA / drug effects
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DNA / genetics*
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DNA Damage
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DNA Modification Methylases / genetics*
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DNA Modification Methylases / metabolism
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DNA Repair
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DNA Repair Enzymes / genetics*
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DNA Repair Enzymes / metabolism
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Female
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Humans
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Male
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Methylation / drug effects
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Micronucleus Tests
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Middle Aged
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MutL Protein Homolog 1
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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Occupational Diseases / epidemiology
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Occupational Diseases / genetics
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Occupational Exposure / adverse effects*
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Polymerase Chain Reaction
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Polymorphism, Genetic*
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Tumor Suppressor Proteins / genetics*
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Tumor Suppressor Proteins / metabolism
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Vinyl Chloride / adverse effects*
Substances
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Adaptor Proteins, Signal Transducing
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MLH1 protein, human
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Nuclear Proteins
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Tumor Suppressor Proteins
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DNA
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DNA Modification Methylases
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MGMT protein, human
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MutL Protein Homolog 1
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DNA Repair Enzymes
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Vinyl Chloride