Abstract
We used recombinant rat dectin-1 proteins to newly establish sandwich ELISA for determining barley β-glucan (BβG). The ELISA method had a working range of 15-4,000 µg/L. Plasma BβG was detectable up to 24 h after an intravenous administration of BβG (1 mg/kg of body weight) to rats. This method may be an effective tool for investigating the immune modulatory effects of BβG.
MeSH terms
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Animals
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Binding, Competitive
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Calibration
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Enzyme-Linked Immunosorbent Assay / methods*
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Enzyme-Linked Immunosorbent Assay / standards
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Hordeum / chemistry*
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Injections, Intravenous
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Lectins, C-Type / chemistry*
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Lectins, C-Type / genetics
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Limit of Detection
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Male
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Protein Binding
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Protein Structure, Tertiary
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Rats
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Rats, Wistar
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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beta-Glucans / blood*
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beta-Glucans / pharmacokinetics
Substances
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Lectins, C-Type
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Recombinant Proteins
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beta-Glucans
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dectin 1