The increasing availability of spatial data inspires the exploration of previously less-studied, yet regionally and nationally important areas, such as the Irrawaddy and Salween River Basins in Southeast Asia. This article documents our experience using global datasets to create environmental basin profiles in these two basins. Our approach draws on the concepts of freshwater vulnerability assessments that guided the selection of indicators. Data on land use, population distribution and fertilizer load were used. The unit of analysis was chosen to distinguish areas with similar bio-geographical characteristics, such as the critical delta areas. Results were further discussed for sub-areas that experience relatively the most pressure in terms of examined indicators within the studied area. The river mouths of both rivers had the most intensive land use and high population density. They are also home to important ecosystems and are sensitive to changes in upstream areas. Our study presents a concise and spatially distributed view of the environmental basin profiles of the Irrawaddy and Salween River Basins. The analysis also provides some interesting methodological insights about the potential of public macro-scale datasets for environmental assessment. The spatial approach allowed the analysis of different indicators, providing a platform for data integration as well as a visually powerful overview of the study area. Yet, the use of macro-scale datasets entails challenges. Despite improvements, the assessment process tends to be driven by the availability and quality of data, rather than by the actual research and management needs. The greatest utility of macro-scale datasets lies-at least in data-poor areas-in larger scale comparative analyses between the basins and their different sub-areas.