Assessment of DNA damage by micellar electrokinetic chromatography

Paolo Fattorini; Giorgio Marrubini; Pierangela Grignani; Solange Sorçaburu-Cigliero; Carlo Previderé

doi:10.1007/978-1-62703-296-4_25

Assessment of DNA damage by micellar electrokinetic chromatography

Methods Mol Biol. 2013:984:341-51. doi: 10.1007/978-1-62703-296-4_25.

Authors

Paolo Fattorini¹, Giorgio Marrubini, Pierangela Grignani, Solange Sorçaburu-Cigliero, Carlo Previderé

Affiliation

¹ Department of Medicine, surgery and Health, University of Trieste, Trieste, Italy. fattorin@univ.trieste.it

PMID: 23386356
DOI: 10.1007/978-1-62703-296-4_25

Abstract

A simple and inexpensive MEKC method, which is able to assess base damage within DNA samples, is illustrated. After heat-acid hydrolysis of the DNA samples, both the percentage of each canonical DNA base and the relative amount of uncanonical DNA bases can be measured. This method is useful for an evaluation of the integrity of PCR templates used in several fields of investigation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Buffers
Calibration
Chromatography, Micellar Electrokinetic Capillary / methods
Chromatography, Micellar Electrokinetic Capillary / standards
DNA / chemistry
DNA / isolation & purification
DNA Damage*
Electrophoresis, Capillary / methods
Forensic Genetics
Humans
Hydrolysis
Limit of Detection
Polymerase Chain Reaction
Reference Standards
Uridine Triphosphate / analogs & derivatives
Uridine Triphosphate / chemistry

Substances

Buffers
5-bromouridine triphosphate
DNA
Uridine Triphosphate