Abstract
Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006-2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucleocapsid (N) genes of 32 representative field strains were determined, and the open reading frames (ORFs) of these N genes were 1326 nucleotides long. The N gene sequences were found to be more than 95 % identical to each other. The number of potential phosphorylation sites in the N protein varied from 5 to 12. A phylogenetic tree based on the N genes showed that the 32 Chinese field strains formed three groups.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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China / epidemiology
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Coronavirus Infections / epidemiology
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Coronavirus Infections / veterinary
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Coronavirus Infections / virology
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Genetic Variation / genetics
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Molecular Sequence Data
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Nucleocapsid / genetics*
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Nucleocapsid Proteins / genetics
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Phylogeny
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Porcine epidemic diarrhea virus / genetics*
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Swine / virology
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Swine Diseases / epidemiology
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Swine Diseases / virology*
Associated data
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GENBANK/FJ473387
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GENBANK/FJ473388
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GENBANK/FJ473389
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GENBANK/FJ473390
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GENBANK/FJ473391
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GENBANK/FJ473392
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GENBANK/FJ473393
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GENBANK/FJ473394
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GENBANK/HM210880
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GENBANK/HM210881
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GENBANK/HM210882
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GENBANK/HQ455345
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GENBANK/HQ455346
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GENBANK/JN601052
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GENBANK/JN601053
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GENBANK/JN601054
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GENBANK/JN601055
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GENBANK/JN601056
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GENBANK/JN601057
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GENBANK/JN601058
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GENBANK/JN601059
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GENBANK/JN601060
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GENBANK/JN601061
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GENBANK/JN601062
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GENBANK/JQ735953
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GENBANK/JQ743650
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GENBANK/JQ743651
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GENBANK/JQ743652
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GENBANK/JQ743653
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GENBANK/JQ743654
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GENBANK/JQ743655
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GENBANK/JQ743656