Kocurin, the true structure of PM181104, an anti-methicillin-resistant Staphylococcus aureus (MRSA) thiazolyl peptide from the marine-derived bacterium Kocuria palustris

Mar Drugs. 2013 Feb 4;11(2):387-98. doi: 10.3390/md11020387.

Abstract

A new thiazolyl peptide, kocurin (1), was isolated from culture broths of a marine-derived Kocuria palustris. Its structural elucidation was accomplished using a combination of spectroscopic and chemical methods, including HRMS, extensive 1D and 2D NMR analysis, MS/MS fragmentation, and chemical degradation and Marfey's analysis of the resulting amino acid residues. The structure herein reported corrects that previously assigned to PM181104 (3). Kocurin displayed activity against methicillin-resistant Staphylococcus aureus (MRSA), with MIC values in the submicromolar range.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacology*
  • Antifungal Agents / chemistry
  • Antifungal Agents / pharmacology
  • Candida albicans / drug effects
  • Gene Expression Regulation, Bacterial / physiology
  • Hydrolysis
  • Methicillin-Resistant Staphylococcus aureus / drug effects*
  • Micrococcaceae / chemistry
  • Micrococcaceae / metabolism*
  • Ozone
  • Peptides / chemistry
  • Peptides / pharmacology*
  • Peptides, Cyclic / chemistry*
  • Peptides, Cyclic / pharmacology*
  • Protein Conformation
  • Thiazoles / chemistry*
  • Thiazoles / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Antifungal Agents
  • Peptides
  • Peptides, Cyclic
  • Thiazoles
  • kocurin
  • Ozone