Abstract
Ninety-nine clinical isolates previously identified as Klebsiella oxytoca were evaluated using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Eight isolates were identified as Raoultella spp., being 5 Raoultella spp. and 3 K. oxytoca, by 16S rRNA sequencing. These isolates were correctly identified by applying the 10% differential rule for the MALDI-TOF MS score values. This approach might be useful to discriminate Raoultella species from K. oxytoca.
Copyright © 2013 Elsevier Inc. All rights reserved.
MeSH terms
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Bacteriological Techniques / methods*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Enterobacteriaceae / chemistry*
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Enterobacteriaceae / classification*
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Humans
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Molecular Sequence Data
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Substances
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S
Associated data
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GENBANK/JX102650
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GENBANK/JX102651
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GENBANK/JX102652
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GENBANK/JX102653
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GENBANK/JX102654