Objective: To evaluate the effect of chitosan (CH) and hydrogen peroxide (H(2)O(2)) seed coatings and seedling sprinklings on two different maize varieties by measuring their phenology, the H(2)O(2) presence, the catalase (CAT) activity, and the protein quantity.
Methods: Seven groups of ten seeds for each maize variety were treated with CH (2% (20 g/L) and 0.2% (2 g/L)) or H(2)O(2) (8 mmol/L) by coating, sprinkling, or both. Germination and seedling growth were measured. One month after germination, the presence of H(2)O(2) in seedlings in the coated seed treatments was evaluated. Protein content and CAT activity were determined under all treatments.
Results: H(2)O(2) seed coating enhanced the germination rate and increased seedling and stem length in the quality protein maize (QPM) variety. Seedlings had a higher emergence velocity under this treatment in both varieties. CH and H(2)O(2) sprinklings did not have an effect on seedling phenology. Exogenous application of H(2)O(2) promoted an increase of endogenous H(2)O(2). CH and H(2)O(2) seedling sprinkling increased the protein content in both maize varieties, while there was no significant effect on the CAT activity of treated seeds and seedlings.
Conclusions: CH and H(2)O(2) enhance some phenological and biochemical features of maize depending on their method of application.