Purpose: To investigate ectopic osteogenesis of composites SGDs with porous calcium phosphate cement(pCPC) in goat's muscle pouch.
Methods: SGDs were cultivated with modified tissue culture techniques, then were induced into osteoblasts in the third passage, the osteogenic-induced SGDs were combined with pCPC and transplanted into the goat left dorsal muscle pouch, the pCPC without cells was transplanted into the right dorsal muscle pouch as negative controls. The transplants were harvested at 2-,4-,6-,8- week and prepared for histological examination. The morphologic quantitative analysis was made by SPSS 16.0 software package.
Results: Bone formation was not detected in pCPC without cells by histological examination. 2,4,6,8 weeks after transplantation in SGDs-pCPC group, the percentages of bone formation were (1.24±0.25)%,(1.59±0.23)%,(4.12±0.39)% and (5.68±0.58)%,respectively.There was no significant difference in bone formation at 2 and 4 weeks after transplantation (P>0.05). At 8- week, the percentages of bone formation were higher than that at 6- week in SGDs-pCPC group, and both significantly higher than that at 2- and 4- week(P<0.05).
Conclusion: SGDs combined with pCPC have the ability of ectopic osteogenesis.