According to the first article of the waste management rule ratified in 2004, healthcare facilities are responsible for sterilizing their own infectious wastes. Nowadays non-incineration techniques are preferred internationally owing to toxic emissions caused by incinerators. Surveys have been conducted in Iran, resulting in ratification of an executive code by the Iranian Board of Ministers, which imposed a ban on incinerators; furthermore, it emphasized, instead, the use of sterilizers. The main objective of this research was evaluating the present status of these facilities to figure out which ones have less effect on the community, personnel and on the environment. A questionnaire was used to collect the data. The results showed that biological tests had not been conducted for most of the sterilizers. As biological testing is the most important factor in assessing autoclave accuracy, a comprehensive program should be initiated to perform these tests to improve functioning of the sterilizers so that the best treating process can be achieved.