Complete sequence of the IncT-type plasmid pT-OXA-181 carrying the blaOXA-181 carbapenemase gene from Citrobacter freundii

Antimicrob Agents Chemother. 2013 Apr;57(4):1965-7. doi: 10.1128/AAC.01297-12. Epub 2013 Jan 28.

Abstract

The gene encoding the carbapenemase OXA-181 (an OXA-48 variant) was identified from a Citrobacter freundii isolate coproducing NDM-1. The whole sequence of plasmid pT-OXA-181 bearing the blaOXA-181 gene was determined and revealed a 84-kb mobilizable but non-self-conjugative IncT-type plasmid. It totally differs from the 7.6-kb ColE-type and blaOXA-181-bearing plasmid recently identified in a Klebsiella pneumoniae isolate. However, in both plasmids, insertion sequence ISEcp1 might have played a role in acquisition of the blaOXA-181 gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Citrobacter freundii / drug effects
  • Citrobacter freundii / enzymology*
  • Citrobacter freundii / genetics
  • Molecular Sequence Data
  • Plasmids / genetics*
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • beta-Lactamases
  • beta-lactamase NDM-1
  • carbapenemase

Associated data

  • GENBANK/JQ996148
  • GENBANK/JQ996149
  • GENBANK/JQ996150