Abstract
A genetically encoded precursor to an aryl nitrene, para-azidophenylalanine, was introduced site specifically into proteins to deduce if distinct environments were capable of caging a reactive organic intermediate. Following photolysis of mutant T4 lysozyme or green fluorescent proteins, EPR spectra showed, respectively, the presence of a triplet nitrene and an anilino radical.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Azides / chemistry*
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Bacteriophage T4 / enzymology
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Bacteriophage T4 / genetics
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Electron Spin Resonance Spectroscopy
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Green Fluorescent Proteins / chemistry
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Imines / chemistry
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Molecular Structure
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Phenylalanine / analogs & derivatives*
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Phenylalanine / chemistry
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Photochemistry
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Photolysis
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Ultraviolet Rays
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Viral Proteins / chemistry
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Viral Proteins / genetics
Substances
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Azides
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Imines
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Viral Proteins
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gene 5 protein, Enterobacteria phage T4
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Green Fluorescent Proteins
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phenylnitrene
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4-azidophenylalanine
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Phenylalanine