Evaluation of IgG anti-toxoplasma avidity and polymerase chain reaction in the postnatal diagnosis of congenital toxoplasmosis

Pediatr Infect Dis J. 2013 Jun;32(6):693-5. doi: 10.1097/INF.0b013e31828807a4.

Abstract

Confirmatory tests for congenital toxoplasmosis were evaluated in 23 infected and 31 uninfected newborns. Conventional polymerase chain reaction was better than real-time polymerase chain reaction, but did not identify additional cases. Avidity tests added 2 new cases that were not identified by other criteria. Overall sensitivity was 82.6%. Avidity assay, but not polymerase chain reaction, increased the sensitivity of confirmatory assays in congenital toxoplasmosis.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Protozoan / blood*
  • Antibody Affinity*
  • Female
  • Humans
  • Immunoassay / methods
  • Immunoglobulin G / blood
  • Infant
  • Infant, Newborn
  • Male
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Toxoplasma / genetics
  • Toxoplasma / immunology
  • Toxoplasma / isolation & purification*
  • Toxoplasmosis, Congenital / diagnosis*

Substances

  • Antibodies, Protozoan
  • Immunoglobulin G