Rapid and simultaneous detection of genes encoding Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) in Gram-negative bacilli

Scott A Cunningham; Tabassum Noorie; Daniele Meunier; Neil Woodford; Robin Patel

doi:10.1128/JCM.03062-12

Rapid and simultaneous detection of genes encoding Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) in Gram-negative bacilli

J Clin Microbiol. 2013 Apr;51(4):1269-71. doi: 10.1128/JCM.03062-12. Epub 2013 Jan 23.

Authors

Scott A Cunningham¹, Tabassum Noorie, Daniele Meunier, Neil Woodford, Robin Patel

Affiliation

¹ Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.

Abstract

We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with reference methods and a turnaround time of 90 min.

Publication types

Evaluation Study

MeSH terms

Bacterial Proteins / genetics*
Bacteriological Techniques / methods*
Gram-Negative Bacteria / enzymology*
Gram-Negative Bacteria / genetics
Gram-Negative Bacteria / isolation & purification
Gram-Negative Bacterial Infections / microbiology
Humans
Multiplex Polymerase Chain Reaction / methods*
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Time Factors
beta-Lactamases / genetics*

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase