Conformational stability of leukocyte lactate dehydrogenase in healthy men of different age

Bull Exp Biol Med. 2012 Nov;154(1):44-6. doi: 10.1007/s10517-012-1871-9.

Abstract

Comparative analysis of cytosolic fraction distribution and conformational stability in lactate dehydrogenase (LDH) isozymes in healthy men of different age revealed significant differences indicating age-related characteristics of the enzyme. Activity of cytosolic fraction of LDH-1 and conformational stability of LDH-2 and LDH-3 in 40-year-old men were higher than in 20-year-olds. These data suggest age-related determination of conformational stability of individual LDH isozymes and functional heterogeneity of the enzyme within each isoform.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aging*
  • Cytosol / chemistry
  • Enzyme Stability
  • Humans
  • Isoenzymes / chemistry
  • Isoenzymes / metabolism
  • L-Lactate Dehydrogenase / chemistry*
  • L-Lactate Dehydrogenase / metabolism
  • Leukocytes / enzymology*
  • Male
  • Middle Aged
  • Protein Conformation
  • Young Adult

Substances

  • Isoenzymes
  • L-Lactate Dehydrogenase
  • lactate dehydrogenase 2
  • lactate dehydrogenase 3
  • lactate dehydrogenase 1